12719 Skor1 Status: ES cell colonies screened / QC no positives
| Gene | Skor1 |
| Chromosome | 9 |
| Deletion Start | 63,146,910 |
| Deletion End | 63,138,781 |
| Deletion Size | 8,130 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,146,502 | 63,146,522 | CCTCTCCCAACTCCAAGCAAG |
| TUP | 63,146,478 | 63,146,498 | TGCAGCCCTACTCGGGATCCA |
| TUR | 63,146,396 | 63,146,415 | CGCTCCTGCCCATCAATGAC |
| TDF | 63,139,604 | 63,139,621 | GCTGTGACCTCAGCCATC |
| TDP | 63,139,579 | 63,139,603 | TGCCTAGAGGGTGGTCTCACAGAAC |
| TDR | 63,139,536 | 63,139,556 | TCCTGGTCCAGTTCGTTACAC |
PCR Primers
| Primer | Sequence |
| SU | CCACGAAGATAGGTGCATTG |
| SD | GCCACGTTGTCGTTAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
