12724 Ube2o Status: Cancelled
| Gene | Ube2o |
| Chromosome | 11 |
| Deletion Start | 116,581,410 |
| Deletion End | 116,548,776 |
| Deletion Size | 32,635 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,580,553 | 116,580,574 | CCTTTGAATTGCCTCGGAGAAC |
| TUP | 116,580,521 | 116,580,548 | TGAGGGAAATGATGAACCAGTCCAGGGC |
| TUR | 116,580,459 | 116,580,481 | CAAGAAGTCCTAGACACCCAGTC |
| TDF | 116,549,420 | 116,549,444 | GCAGGCAGAATAGCCAGATTAGAAG |
| TDP | 116,549,391 | 116,549,417 | TTGTGTCTAGTGACAGCTGGCTTCAGT |
| TDR | 116,549,355 | 116,549,375 | ATGGGTGCTCAGGAGTGGTTG |
PCR Primers
| Primer | Sequence |
| SU | AACTCCGGTAGACCAGTACCC |
| SD | CCGTGTCTGTGGTCAATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
