| Gene | Zfp867 |
| Chromosome | 11 |
| Deletion Start | 59,472,364 |
| Deletion End | 59,462,985 |
| Deletion Size | 9,380 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,472,118 | 59,472,136 | GACGCTGGCTTCTTGGTTG |
| TUP | 59,472,098 | 59,472,117 | TGGGTTCCCGCCCTTCAGCC |
| TUR | 59,472,038 | 59,472,062 | GCAGGCGAAGAGTAAATAGATTGAG |
| TDF | 59,463,170 | 59,463,190 | GCATTCAGTTGCTCCACTTCC |
| TDP | 59,463,140 | 59,463,168 | TTAGGAGACACGAACAGGCTCATAGTGGG |
| TDR | 59,463,104 | 59,463,127 | CAAAGGCTTTCCCACACTGTATTC |
PCR Primers
| Primer | Sequence |
| SU | AGGGCCATTGCTAGAGTGC |
| SD | CTCTGATGATATGCTGTCCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12735A-C2 | VGB6 | C57BL/6NTac | |
| 12735A-C10 | VGB6 | C57BL/6NTac | |
| 12735A-H9 | VGB6 | C57BL/6NTac | |
| 12735A-H11 | VGB6 | C57BL/6NTac | |
