12741 Cd300lh Status: Cancelled
| Gene | Cd300lh |
| Chromosome | 11 |
| Deletion Start | 115,048,292 |
| Deletion End | 115,041,725 |
| Deletion Size | 6,568 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,047,998 | 115,048,018 | CCTAGCTGCTCCATGAAAGCT |
| TUP | 114,989,470 | 114,989,495 | TTTGGGTTTCATGTTTGGCACATGCA |
| TUR | 115,047,827 | 115,047,852 | GTGTCAACTAAAGCTCACAATGGAAT |
| TDF | 115,020,502 | 115,020,522 | CAACTTGGCAAGGTGAAGGTG |
| TDP | | | TGAGAAACCCTGTCTC |
| TDR | 115,042,091 | 115,042,111 | CCCTCTCCCTCTCCTTCTCTG |
PCR Primers
| Primer | Sequence |
| SU | GCAGCTCAGTTACCTCCCAG |
| SD | GCTATCCAGTGAGACAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 192 bp | + | + | - |
| TDF | TDR | 21610 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
