12742 Apol10a Status: ES cell colonies screened / QC positives
| Gene | Apol10a |
| Chromosome | 15 |
| Deletion Start | 77,311,693 |
| Deletion End | 77,319,785 |
| Deletion Size | 8,093 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,311,847 | 77,311,870 | TGGTCATTGTGCATAAGATGAGTG |
| TUP | 77,311,889 | 77,311,917 | TCATGTACTTGAGCCCTTGGATTCCAGTT |
| TUR | 77,311,942 | 77,311,963 | CGCTCCATGTCCTGTGAGTTAC |
| TDF | 77,319,387 | 77,319,412 | GATGATCAGTAGAGCCAGGATTAGAG |
| TDP | 77,319,417 | 77,319,443 | AGGGCTTACAGTTCCGTTCCTTGAACA |
| TDR | 77,319,461 | 77,319,484 | CCTTCAAAACCTCGAATTGTTGCG |
PCR Primers
| Primer | Sequence |
| SU | TGATGCTGGTCCCGTAATAAG |
| SD | GCCTTCTACATTGACCTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12742A-C3 | VGB6 | C57BL/6NTac | |
| 12742A-C5 | VGB6 | C57BL/6NTac | |
| 12742A-C6 | VGB6 | C57BL/6NTac | |
| 12742A-D2 | VGB6 | C57BL/6NTac | |
| 12742A-G9 | VGB6 | C57BL/6NTac | |
| 12742A-G12 | VGB6 | C57BL/6NTac | |
