12760 Fbp1 Status: Cancelled
| Gene | Fbp1 |
| Chromosome | 13 |
| Deletion Start | 62,888,040 |
| Deletion End | 62,864,904 |
| Deletion Size | 23,137 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,887,914 | 62,887,933 | GCTCTGCACCGCGATCAAAG |
| TUP | 62,887,891 | 62,887,910 | TCTCGTCTGCGGTGCGCCAG |
| TUR | 62,887,820 | 62,887,840 | GCAGACCCCAGTCGTTCTAAC |
| TDF | 62,865,176 | 62,865,196 | GCCTTGGTGCCTCTGATCTTG |
| TDP | 62,865,154 | 62,865,174 | TTTCCAGCTGCGGCTGCTGTA |
| TDR | 62,865,113 | 62,865,131 | CACCGGCCTTCTCCATGAC |
PCR Primers
| Primer | Sequence |
| SU | GGGTTATGGGAGGAGGAGTC |
| SD | CCCAGTTTATGGCATGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
