12768 Ccl2 Status: Cancelled
| Gene | Ccl2 |
| Chromosome | 11 |
| Deletion Start | 82,035,665 |
| Deletion End | 82,037,263 |
| Deletion Size | 1,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,036,064 | 82,036,085 | TCCAAGATCCGAGCTGTGTTTC |
| TUP | 82,036,088 | 82,036,111 | CCAGCCCTGCTTCCAGAGATAGCA |
| TUR | 82,036,113 | 82,036,132 | TCCCAACCACCTCCGCTAAG |
| TDF | 82,037,129 | 82,037,149 | CAACCACCTCAAGCACTTCTG |
| TDP | 82,037,151 | 82,037,179 | AGGAGTGACCAGTGTGACAGTGAACTAGT |
| TDR | 82,037,180 | 82,037,199 | GGCATCACAGTCCGAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GGCAAGCTAGGCTGTGTTTC |
| SD | GAGAAGCAATGCTAGGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
