12770   Il18bp     Status: ES cell clone microinjected

GeneIl18bp
Chromosome7
Deletion Start102,017,311
Deletion End102,015,739
Deletion Size1,573
Genome Build38
MGI IDMGI:1333800
NCBI Gene ID16068
BAC ID383B20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF102,016,595102,016,617TGTCTCTGTGTTCACCATTCTAC
TUP102,016,571102,016,594TGCCCAGCTTCCCAGATGGTTCCT
TUR102,016,543102,016,567CATTGCCTATGTCTGAGTTCTCTTC
TDF102,016,057102,016,077TGGCCCAGTATCACATCATTC
TDP102,016,034102,016,056TGGCCCAGCTCTGGGTAAGGAAC
TDR102,015,997102,016,015AGTAGCGTTCCTCCCATCC

PCR Primers

PrimerSequence
SUCCTAGAAGCTGACTGCTGCC
SDTGGTCAGAGCTGAGTGGATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12770A-C8VGB6C57BL/6NTac
12770A-F7VGB6C57BL/6NTac
12770A-F3VGB6C57BL/6NTac
12770A-D7VGB6C57BL/6NTac