12775 Lrrtm4 Status: ES cell clone microinjected
| Gene | Lrrtm4 |
| Chromosome | 6 |
| Deletion Start | 80,022,546 |
| Deletion End | 80,023,181 |
| Deletion Size | 636 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,022,662 | 80,022,683 | CGATGCCGTGGAGACTTACAAC |
| TUP | 80,022,687 | 80,022,711 | TGCTCTGATGTCCAGGTGGTCAACA |
| TUR | 80,022,714 | 80,022,732 | GCGCCAGATGTGATCGTTC |
| TDF | 80,022,923 | 80,022,946 | GATTCTCCTGGTGATCTATGTGTC |
| TDP | 80,022,948 | 80,022,970 | TGGAAACGGTACCCAGCCAGCAT |
| TDR | 80,022,971 | 80,022,992 | TGAGTGCTGTTGGAGTTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCTTCAACCTGCGATCC |
| SD | TCATGTTTCTAAGTCCAACCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
