12776 Adrb1 Status: Cancelled
| Gene | Adrb1 |
| Chromosome | 19 |
| Deletion Start | 56,722,375 |
| Deletion End | 56,723,838 |
| Deletion Size | 1,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,723,015 | 56,723,034 | GCTGCGATTTCGTCACCAAC |
| TUP | 56,723,035 | 56,723,056 | AGGGCCTACGCCATCGCCTCGT |
| TUR | 56,723,080 | 56,723,098 | CGAAGGCCATGATGCACAG |
| TDF | 56,723,689 | 56,723,707 | AACGGCGGGACAACCACTG |
| TDP | 56,723,708 | 56,723,729 | TGGACAGCGATTCGAGCCTGGA |
| TDR | 56,723,763 | 56,723,783 | AGCCTGGCTCTCTACACCTTG |
PCR Primers
| Primer | Sequence |
| SU | TCTTTGGAGCCTTTCAATCG |
| SD | TGATAGACTCCATCCTGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
