12777 Adrb2 Status: ES cell colonies screened / QC no positives
| Gene | Adrb2 |
| Chromosome | 18 |
| Deletion Start | 62,179,749 |
| Deletion End | 62,178,472 |
| Deletion Size | 1,278 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,179,650 | 62,179,667 | ACGAAGCGTGGGTTGTGG |
| TUP | 62,179,616 | 62,179,643 | CCATCCTCATGTCGGTTATCGTCCTGGC |
| TUR | 62,179,569 | 62,179,588 | CGAACTTGGCAATGGCTGTG |
| TDF | 62,178,571 | 62,178,590 | GCATGGAAGGCTTTGTGAAC |
| TDP | 62,178,543 | 62,178,568 | TCAAGGTACTGTGCCTAGCCTTAGCG |
| TDR | 62,178,492 | 62,178,515 | TGTATTACAGTGGCGAGTCATTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCTAGGGTAGCTGGAAGGG |
| SD | TGGAGTGAGAGAGAAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
