12778 Sry Status: ES cell colonies screened / QC no positives
| Gene | Sry |
| Chromosome | Y |
| Deletion Start | 2,663,655 |
| Deletion End | 2,662,457 |
| Deletion Size | 1,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome Y
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 2,663,400 | 2,663,422 | GGCTAAAGTGTCACAGAGGAGTG |
| TUP | 2,663,371 | 2,663,395 | TTTACAGCCTGCAGTTGCCTCAACA |
| TUR | 2,663,307 | 2,663,329 | TCCAGTCTTGCCTGTATGTGATG |
| TDF | 2,662,556 | 2,662,574 | TCCACGACCAGCAGCTTAC |
| TDP | 2,662,523 | 2,662,549 | ACTAACAGCTGACATCACTGGTGAGCA |
| TDR | 2,662,498 | 2,662,517 | GCTGAGGTGCTCCTGGTATG |
PCR Primers
| Primer | Sequence |
| SU | CTAGGTTCATGAGATGGCTTC |
| SD | CATGGCATGCTGTATTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
