12780 Hamp2 Status: Cancelled
| Gene | Hamp2 |
| Chromosome | 7 |
| Deletion Start | 30,924,132 |
| Deletion End | 30,922,507 |
| Deletion Size | 1,626 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,924,052 | 30,924,071 | GCAGCACCACCTATCTCCAG |
| TUP | 30,924,030 | 30,924,051 | CAACAGGTGAGCACCCCAGGCC |
| TUR | 30,923,937 | 30,923,955 | CTTGTCAGCAGCTGCCATG |
| TDF | 30,922,610 | 30,922,639 | GCTTCTTACCTCTCTTTCTTTACACAGATC |
| TDP | 30,922,591 | 30,922,609 | CCAATGCAGAAGAGAAGGA |
| TDR | 30,922,541 | 30,922,563 | GTTACAGCACTGACAGCAGAATC |
PCR Primers
| Primer | Sequence |
| SU | AACAGCCATACTGAGGGCAC |
| SD | TCTGGCTCTTCCATGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
