12787 Tbx6 Status: Cancelled
| Gene | Tbx6 |
| Chromosome | 7 |
| Deletion Start | 126,781,510 |
| Deletion End | 126,785,230 |
| Deletion Size | 3,721 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,782,028 | 126,782,050 | CAACCCAGACAGCAGAATCTAAC |
| TUP | 126,782,052 | 126,782,074 | ATGGCCTGACACTGTGACCCTGG |
| TUR | 126,782,098 | 126,782,119 | GCCAGCCTGATCCTTAAACCTC |
| TDF | 126,785,120 | 126,785,139 | CCCAGGACCTGGAGGTTATC |
| TDP | 126,785,146 | 126,785,171 | TGGGATCCAAGCCAATGTACTGAGCC |
| TDR | 126,785,192 | 126,785,213 | GCTGGAGGATCAAGGGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | AACTGCGTCCCTGTCTTAGC |
| SD | GCTGGACCTTAAAGCTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
