| Gene | Cdkn1c |
| Chromosome | 7 |
| Deletion Start | 143,460,665 |
| Deletion End | 143,459,021 |
| Deletion Size | 1,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,460,510 | 143,460,528 | GAACCGCTGGGACTTCAAC |
| TUP | 143,460,485 | 143,460,508 | TCCAGCAGGATGTGCCTCTTCGAG |
| TUR | 143,460,443 | 143,460,461 | CAGACTCGCTGTCCACCTC |
| TDF | 143,459,246 | 143,459,263 | TTGTCGCCCGCAGACTTC |
| TDP | 143,459,223 | 143,459,245 | TTCGCCAAGCGCAAGAGAACTGC |
| TDR | 143,459,199 | 143,459,216 | TCGTTCGACGCCTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTATGCTAATCGTGAGGAGGC |
| SD | GTGGCAGAGGGATCCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12789B-A2 | VGB6 | C57BL/6NTac | |
| 12789B-A6 | VGB6 | C57BL/6NTac | |
| 12789B-G10 | VGB6 | C57BL/6NTac | |
| 12789B-D11 | VGB6 | C57BL/6NTac | |
| 12789B-G3 | VGB6 | C57BL/6NTac | |
| 12789B-H2 | VGB6 | C57BL/6NTac | |
