12790 Atoh1 Status: Cancelled
| Gene | Atoh1 |
| Chromosome | 6 |
| Deletion Start | 64,729,326 |
| Deletion End | 64,730,399 |
| Deletion Size | 1,074 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,729,620 | 64,729,642 | CAGGGTGAGCTGGTAAGGAGAAG |
| TUP | 64,729,646 | 64,729,667 | CTGTGGCGGCCTCAGCAAGAGC |
| TUR | 64,729,716 | 64,729,737 | AGCCAAGCTCGTCCACTACAAC |
| TDF | 64,729,872 | 64,729,892 | CCGTCCTTCAACAACGACAAG |
| TDP | 64,729,900 | 64,729,927 | CCAAATATGAGACCCTACAGATGGCCCA |
| TDR | 64,729,931 | 64,729,951 | TCCGACAGAGCGTTGATGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTTTGACTGGGCAGACAC |
| SD | TAAAGTACCCAATGCGGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
