| Gene | Lta |
| Chromosome | 17 |
| Deletion Start | 35,204,650 |
| Deletion End | 35,203,694 |
| Deletion Size | 957 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,204,427 | 35,204,445 | CAGGACAGCCCATCCACTC |
| TUP | 35,204,402 | 35,204,426 | CCTCAGAAGCACTTGACCCATGGCA |
| TUR | 35,204,378 | 35,204,397 | CAAGGTGAGCAGCAGGTTTC |
| TDF | 35,204,124 | 35,204,144 | CCCAGCAAGCAGAACTCACTG |
| TDP | 35,204,100 | 35,204,122 | TCTGGAGAGCAAGCACGGATCGT |
| TDR | 35,204,056 | 35,204,079 | AGGAGGGAGTTGTTGCTCAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGTTAGCCATTGTCTG |
| SD | ACACCTTTGTGTCTGGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12792A-A6 | VGB6 | C57BL/6NTac | |
| 12792A-B1 | VGB6 | C57BL/6NTac | |
| 12792A-B9 | VGB6 | C57BL/6NTac | |
| 12792A-E2 | VGB6 | C57BL/6NTac | |
