12799 Kcnj11 Status: Cancelled
| Gene | Kcnj11 |
| Chromosome | 7 |
| Deletion Start | 46,099,894 |
| Deletion End | 46,098,704 |
| Deletion Size | 1,191 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,099,773 | 46,099,793 | CGTGTCCAAGAAAGGCAACTG |
| TUP | 46,099,748 | 46,099,772 | CAACGTCGCCCACAAGAACATTCGA |
| TUR | 46,099,708 | 46,099,726 | CCAGCGTGGTGAACACATC |
| TDF | 46,098,908 | 46,098,927 | GCCGCTATTCTGTGGACTAC |
| TDP | 46,098,872 | 46,098,899 | TGGTAACACCATTAAAGTGCCCACACCA |
| TDR | 46,098,848 | 46,098,866 | ATCAAGCTGGCGGGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GGACAGAAGTGGGTGAGGTC |
| SD | TACTTCAGCATGGCACGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
