| Gene | Rhob |
| Chromosome | 12 |
| Deletion Start | 8,499,629 |
| Deletion End | 8,498,946 |
| Deletion Size | 684 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,499,558 | 8,499,577 | CGTGCCTGCTGATCGTGTTC |
| TUP | 8,499,520 | 8,499,540 | CGAGGTGTACGTGCCCACCGT |
| TUR | 8,499,494 | 8,499,513 | CGATGTCCGCCACATAGTTC |
| TDF | 8,499,216 | 8,499,235 | GCATGAAGCAGGAGCCAGTG |
| TDP | 8,499,193 | 8,499,212 | ACGGATGACGGCCGCGCCAT |
| TDR | 8,499,160 | 8,499,183 | TCGAGGTAGTCATAGGCTTGGATG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTAGGTCTAGAGTGAAC |
| SD | GCAACAGTAGTGGCTTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12801A-D10 | VGB6 | C57BL/6NTac | |
| 12801A-G9 | VGB6 | C57BL/6NTac | |
