12808 Chrm5 Status: ES cell colonies screened / QC one positive
| Gene | Chrm5 |
| Chromosome | 2 |
| Deletion Start | 112,480,766 |
| Deletion End | 112,479,078 |
| Deletion Size | 1,689 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,480,150 | 112,480,172 | GCCGCTTTCTATATCCCTGTCTC |
| TUP | 112,480,117 | 112,480,144 | TGACCATACTCTACTGCCGGATCTACCG |
| TUR | 112,480,089 | 112,480,109 | CAGGTCCTTGGTTCGTTTCTC |
| TDF | 112,479,856 | 112,479,878 | GAGCAGGTTACCAACTGTAGCAG |
| TDP | 112,479,826 | 112,479,850 | CCTCTTCAGAGGACGAAGCCAAGGC |
| TDR | 112,479,773 | 112,479,792 | GGCTTTCCTTGGCCTCATTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTATCCAATGCAATGATGTG |
| SD | GGCTTACACGCTCCTGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
