12817 Nkx6-1 Status: ES cell colonies screened / QC no positives
| Gene | Nkx6-1 |
| Chromosome | 5 |
| Deletion Start | 101,664,231 |
| Deletion End | 101,659,359 |
| Deletion Size | 4,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,663,339 | 101,663,358 | GGGCTCCACCAACGAGAATG |
| TUP | 101,663,315 | 101,663,338 | CGTCTGGACAGAACACAGCGCAGC |
| TUR | 101,663,249 | 101,663,269 | CCCGCTTTCCATCCATCTGAC |
| TDF | 101,659,845 | 101,659,863 | TGGCCTCTGTGAGCTTGTG |
| TDP | 101,659,820 | 101,659,843 | TACTATCGCTATGGCAACGGCGGT |
| TDR | 101,659,779 | 101,659,797 | GGGTCCAGCCGTCAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | TACGTCTTGCTGATTTCCCG |
| SD | CTCGGCTCCGAGATTCTCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
