12818 Endog Status: ES cell colonies screened / QC positives
| Gene | Endog |
| Chromosome | 2 |
| Deletion Start | 30,027,098 |
| Deletion End | 30,029,586 |
| Deletion Size | 2,489 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGCAGGGTCTTGATTGTGAAG |
| TUP | | | AGGTTCCTGGGACATCCACCCA |
| TUR | | | TGGTCTCCTGTCCATCTCCATC |
| TDF | | | CCACGTCTGACCTGCCTTAC |
| TDP | | | ACCTGTAACCCGTGGCTGCACGG |
| TDR | | | GCTCATTTGTCTGCCAGGAATC |
PCR Primers
| Primer | Sequence |
| SU | ACATACTTCCCAGGCCAAAC |
| SD | CACCCATCCTGGAGACTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12818A-E1 | VGB6 | C57BL/6NTac | |
| 12818A-F4 | VGB6 | C57BL/6NTac | |
| 12818A-E5 | VGB6 | C57BL/6NTac | |
