12819 Cldn5 Status: ES cell colonies screened / QC positives
| Gene | Cldn5 |
| Chromosome | 16 |
| Deletion Start | 18,777,092 |
| Deletion End | 18,777,807 |
| Deletion Size | 716 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGTGGCACTCTTTGTTACCTTG |
| TUP | | | ACCGGCGCTCAGTGCACCAC |
| TUR | | | TGCTACCCGTGCCTTAACTG |
| TDF | | | AACATCGTTGTCCGCGAGTTC |
| TDP | | | TATGATCCGACGGTGCCGGTGTC |
| TDR | | | CGCCCAGCTCGTACTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGGTCATGCTGCTAACAGTG |
| SD | CTGCCCTTTCAGGTTAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12819A-A5 | VGB6 | C57BL/6NTac | |
| 12819A-C6 | VGB6 | C57BL/6NTac | |
| 12819A-E2 | VGB6 | C57BL/6NTac | |
| 12819A-D6 | VGB6 | C57BL/6NTac | |
