12826 Mab21l1 Status: Cancelled
| Gene | Mab21l1 |
| Chromosome | 3 |
| Deletion Start | 55,782,997 |
| Deletion End | 55,784,084 |
| Deletion Size | 1,088 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,783,085 | 55,783,104 | TCCGGGAAGTCTGCAAAGTG |
| TUP | 55,783,107 | 55,783,130 | CTCCGACGTCCTGAAGGAGGTTGA |
| TUR | 55,783,165 | 55,783,184 | TCGTAGCGGTTGTCCATCTC |
| TDF | 55,783,797 | 55,783,820 | CCTGAACAACTACCACATGAAGAC |
| TDP | 55,783,821 | 55,783,844 | TCTGGTGTCCTACGAGTGCGAGAA |
| TDR | 55,783,853 | 55,783,872 | TTCGTCCCAGTCCGACTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGCTCTTACACAAACCAAC |
| SD | ATCCTTTGCTGGAGTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
