12834 Foxc1 Status: ES cell colonies screened / QC no positives
| Gene | Foxc1 |
| Chromosome | 13 |
| Deletion Start | 31,807,211 |
| Deletion End | 31,808,888 |
| Deletion Size | 1,678 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,807,882 | 31,807,903 | CCAGGACATCAAGACGGAGAAC |
| TUP | 31,807,906 | 31,807,927 | TACGTGTCCCTCGCCTCCCCAG |
| TUR | 31,807,970 | 31,807,990 | GCTCTCGATTTTGGGCACTGT |
| TDF | 31,808,720 | 31,808,737 | CACTCGGTGCGGGAAATG |
| TDP | 31,808,738 | 31,808,761 | TTCGAGTCTCAGCGGATCGGCTTG |
| TDR | 31,808,802 | 31,808,822 | GAGACTGACTGGCAGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GACTGCGCCTGGAAGTTGATC |
| SD | AGCAGGAAAGCGAGTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
