| Gene | Foxc2 |
| Chromosome | 8 |
| Deletion Start | 121,116,618 |
| Deletion End | 121,118,101 |
| Deletion Size | 1,484 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,116,930 | 121,116,950 | CCCTTCTACCGCGAGAACAAG |
| TUP | 121,116,952 | 121,116,974 | AGGGCTGGCAGAACAGCATCCGC |
| TUR | 121,116,989 | 121,117,011 | GCACTTTCACGAAGCACTCATTG |
| TDF | 121,117,939 | 121,117,958 | CCAACGTCCGGGAGATGTTC |
| TDP | 121,117,963 | 121,117,984 | CGCACCGGCTAGGACTGGACAA |
| TDR | 121,118,033 | 121,118,051 | GGACGGCGTAGCTCGATAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTAGCACTCGGAAGGGC |
| SD | TATCCGGAGAGACCTCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12836A-E12 | VGB6 | C57BL/6NTac | |
| 12836A-F9 | VGB6 | C57BL/6NTac | |
| 12836A-D4 | VGB6 | C57BL/6NTac | |
