12842 Rps11 Status: ES cell colonies screened / QC no positives
| Gene | Rps11 |
| Chromosome | 7 |
| Deletion Start | 45,124,308 |
| Deletion End | 45,122,354 |
| Deletion Size | 1,955 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,124,225 | 45,124,244 | TGGCGTCCTCGTTGTCTTAC |
| TUP | 45,124,198 | 45,124,223 | CATTAGCTGAAGCGAGGATGCCTGCG |
| TUR | 45,124,155 | 45,124,173 | TGCGTAGAGCCCGGATTTC |
| TDF | 45,122,971 | 45,122,993 | TCCTACCTGCTAACACCTTTCTG |
| TDP | 45,122,946 | 45,122,969 | TACTCTCCCAGGTGTCGTGACGAA |
| TDR | 45,122,910 | 45,122,927 | TCCCGGCGGATGACAATG |
PCR Primers
| Primer | Sequence |
| SU | TGAATCCCAGCACGTGTTTC |
| SD | CTGAAGGGTTCCTGTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
