12848 Atoh7 Status: ES cell colonies screened / QC no positives
| Gene | Atoh7 |
| Chromosome | 10 |
| Deletion Start | 63,100,159 |
| Deletion End | 63,100,612 |
| Deletion Size | 454 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCTGCAAACCCCACG |
| TUP | 63,100,183 | 63,100,200 | CCTCCGGCGGGAGCTCGC |
| TUR | | | CGTTGGCCGCCAGACG |
| TDF | 63,100,416 | 63,100,436 | CAGCTACATCATCGCGCTCAC |
| TDP | 63,100,440 | 63,100,461 | CATCCTAGCCGAAGCCGAGCGG |
| TDR | 63,100,523 | 63,100,543 | GGTCTACCTGGAGCCTAGCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTTTCCCAGAGAACTG |
| SD | CCCAATAGGGACAAACTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
