12850 Rpl36 Status: Cancelled
| Gene | Rpl36 |
| Chromosome | 17 |
| Deletion Start | 56,613,591 |
| Deletion End | 56,614,264 |
| Deletion Size | 674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,613,770 | 56,613,792 | GAATGGGAAGGTCTGAGTGGTTC |
| TUP | 56,613,798 | 56,613,819 | CCCGGTCGTTGATTGGGCGTCT |
| TUR | 56,613,820 | 56,613,839 | ACCGACCGACTCCCAGTCTC |
| TDF | 56,614,097 | 56,614,116 | TGGGTGACATGCGACTACTC |
| TDP | 56,614,117 | 56,614,137 | CTCGCAGGTGGGCACGCACAT |
| TDR | 56,614,144 | 56,614,163 | GCTCCTCCCGCTTTCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GTGAGGCAGCATTCCAACTC |
| SD | GTGGCATCAAGGAGAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
