12852 Sit1 Status: ES cell colonies screened / QC positives
| Gene | Sit1 |
| Chromosome | 4 |
| Deletion Start | 43,496,485 |
| Deletion End | 43,495,490 |
| Deletion Size | 996 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GACGGTGCTGCTTCTCATC |
| TUP | | | TCATTGGCTGCACTCTTGTCCCAGT |
| TUR | | | GTCCCTCCTGGTTCCTTCTC |
| TDF | | | TCATCAGATTGCTGTGCCTTTG |
| TDP | | | TCCCAGGTCGGCTGTCTCAAGA |
| TDR | | | TGGTGGGTCCTGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTCTTCAGAGTCTCCGTGG |
| SD | GGCTTCTACCCACTTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
