| Gene | Sit1 |
| Chromosome | 4 |
| Deletion Start | 43,483,580 |
| Deletion End | 43,482,585 |
| Deletion Size | 996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,483,346 | 43,483,364 | GACGGTGCTGCTTCTCATC |
| TUP | 43,483,321 | 43,483,345 | TCATTGGCTGCACTCTTGTCCCAGT |
| TUR | 43,483,288 | 43,483,307 | GTCCCTCCTGGTTCCTTCTC |
| TDF | 43,483,010 | 43,483,031 | TCATCAGATTGCTGTGCCTTTG |
| TDP | 43,482,988 | 43,483,009 | TCCCAGGTCGGCTGTCTCAAGA |
| TDR | 43,482,960 | 43,482,977 | TGGTGGGTCCTGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTCTTCAGAGTCTCCGTGG |
| SD | GGCTTCTACCCACTTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12852A-H8 | VGB6 | C57BL/6NTac | |
| 12852B-A9 | VGB6 | C57BL/6NTac | |
