12855   1700029P11Rik     Status: ES cell colonies screened / QC positives

Gene1700029P11Rik
Chromosome15
Deletion Start81,810,993
Deletion End81,812,885
Deletion Size1,893
Genome Build37
MGI IDMGI:1913596
NCBI Gene ID66346
BAC ID266H7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCGAAACTCTGTGGGCTCTAGTC
TUPTTCACCTTGTGGTCTGCTTACTTCCTGG
TURGCCCAGACATTGGCAAGTATTC
TDFGATGAGTTTTGCCTTCCTTGAAC
TDPAGATCCAAAGTTAACCTGTAAGGCCGGCCT
TDRAAACTACAACTCCCAGCATTCCA

PCR Primers

PrimerSequence
SUTCTTACATCTCTGCTGGTTG
SDAGGAGTAACCAGTGAACCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12855A-D3VGB6C57BL/6NTac
12855A-E6VGB6C57BL/6NTac
12855A-D9VGB6C57BL/6NTac
12855A-D8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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