12857   4921510H08Rik     Status: ES cell colonies screened / QC positives

Gene4921510H08Rik
Chromosome10
Deletion Start97,693,480
Deletion End97,694,711
Deletion Size1,232
Genome Build38
MGI IDMGI:1913966
NCBI Gene ID66716
BAC ID63C2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,693,53197,693,551TGGGCATCCTCCATTCCATTG
TUP97,693,55497,693,579CACCTGGTCCTCCTACCATCGAAGGC
TUR97,693,64797,693,667CGCTGTTGCTTGGGTTGTTTC
TDF97,694,10597,694,124AGGCAGCATTGAGGGCACAG
TDP97,694,12597,694,147CAAGCCCAGGAGGGTGGCATCTC
TDR97,694,19197,694,211CGGCAACTGCGAATCTTCTTC

PCR Primers

PrimerSequence
SUGGGAGTGGCTCTCACATCTC
SDACATCCACCTTGAGCATGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR137 bp++-
TDFTDR107 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12857A-H6VGB6C57BL/6NTac
12857A-C2VGB6C57BL/6NTac
12857A-E2VGB6C57BL/6NTac
12857A-G10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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