12859 Dnajb7 Status: ES cell colonies screened / QC positives
| Gene | Dnajb7 |
| Chromosome | 15 |
| Deletion Start | 81,238,567 |
| Deletion End | 81,237,629 |
| Deletion Size | 939 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ACGGCAAGGAAGGATTAGACG |
| TUP | | | CAGAGGTGCAAGCCATCTCGACGA |
| TUR | | | CCGGAATGTGAAGCGGTACTC |
| TDF | | | TCCTACAGCTCCTCAAATACAAC |
| TDP | | | ACTCTGGTGGACAACAATGAACAAGGAACA |
| TDR | | | CCTGCTGAGAAAATGGAAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GACACCATAATGCAGCTCTG |
| SD | TCTGTGCTCAAACTGCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12859A-A2 | VGB6 | C57BL/6NTac | |
| 12859A-A5 | VGB6 | C57BL/6NTac | |
| 12859A-D10 | VGB6 | C57BL/6NTac | |
| 12859A-C3 | VGB6 | C57BL/6NTac | |
| 12859A-A7 | VGB6 | C57BL/6NTac | |
| 12859A-D12 | VGB6 | C57BL/6NTac | |
