12865 Atxn7l3b Status: Cancelled
| Gene | Atxn7l3b |
| Chromosome | 10 |
| Deletion Start | 112,928,719 |
| Deletion End | 112,928,171 |
| Deletion Size | 549 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,928,576 | 112,928,597 | GTGTGGCTACTTCTACCTGGAG |
| TUP | 112,928,553 | 112,928,575 | TTTGCAGACACTGGGAGCGTGAA |
| TUR | 112,928,531 | 112,928,551 | CACTGGCTGGATGCCGAAATC |
| TDF | 112,928,416 | 112,928,435 | CCAGTAGTCACCACCTGAGA |
| TDP | 112,928,391 | 112,928,414 | AGGGCGACACAGTGACCAGGACAA |
| TDR | 112,928,343 | 112,928,367 | CTCCAACTTAGCTTATTCCTCCAAA |
PCR Primers
| Primer | Sequence |
| SU | CTGTCAGCGAGAGGAAGAGG |
| SD | CCACCGAAGCTGACTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
