12874 1700019M22Rik Status: Cancelled
| Gene | 1700019M22Rik |
| Chromosome | 12 |
| Deletion Start | 96,047,117 |
| Deletion End | 96,046,748 |
| Deletion Size | 370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,047,056 | 96,047,076 | TGAGCCATCAACCTCGACATC |
| TUP | 96,047,026 | 96,047,052 | ACCCAAGACAGTGAAGGCTTGCCATCG |
| TUR | 96,046,980 | 96,047,001 | CACCGTGGGCCTTCTTATCTTC |
| TDF | 96,046,848 | 96,046,867 | TTGGTCACTACCACCGTTTG |
| TDP | 96,046,820 | 96,046,847 | TTGAAAGAGCTGTCAACAGGTAGTGGGA |
| TDR | 96,046,790 | 96,046,811 | AGCTCTCAGTGCTGCTATGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCCACATGTTCCATCACAAC |
| SD | GGACAGAGTTTGGTGCAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
