| Gene | Krtap26-1 |
| Chromosome | 16 |
| Deletion Start | 88,647,728 |
| Deletion End | 88,647,030 |
| Deletion Size | 699 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,647,606 | 88,647,625 | ACATGGGCTGTGGAGAGGTC |
| TUP | 88,647,573 | 88,647,596 | CCCAGCAGCTGTCAAGATCACACC |
| TUR | 88,647,529 | 88,647,549 | CTGAGAGGTTCTGCAAAGGTC |
| TDF | 88,647,191 | 88,647,211 | GCCCTCAGACCATTCACATTG |
| TDP | 88,647,164 | 88,647,188 | TCAAACAGCCTGAGACCTCTGCAGC |
| TDR | 88,647,109 | 88,647,129 | GAAGGACGACAAGTGCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CGATGGAAGGTATTTCAGGG |
| SD | AAATAATAAGCCATTGCCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12875A-C1 | VGB6 | C57BL/6NTac | |
| 12875A-D5 | VGB6 | C57BL/6NTac | |
| 12875A-F6 | VGB6 | C57BL/6NTac | |
