12877 2310057N15Rik Status: ES cell colonies screened / QC no positives
| Gene | 2310057N15Rik |
| Chromosome | 16 |
| Deletion Start | 88,774,147 |
| Deletion End | 88,773,499 |
| Deletion Size | 649 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGCTGCCAGGAGACCTA |
| TUP | 88,751,369 | 88,751,389 | CATTGAGCCCATCCGCTGCCA |
| TUR | | | GCACCACACAGGACCTC |
| TDF | 88,773,661 | 88,773,681 | TCAGATCCCTGGATTGTGGAG |
| TDP | 88,773,630 | 88,773,656 | TGGCTTCCCTTCCCTGAGTTATGGGTC |
| TDR | 88,773,600 | 88,773,623 | GAAGCCACATACACTGGGTAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGGAACACAAAGTGATGCTTG |
| SD | GCAATTCAAATCGCAATCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
