| Gene | 1810026J23Rik |
| Chromosome | 9 |
| Deletion Start | 21,592,962 |
| Deletion End | 21,593,872 |
| Deletion Size | 911 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,593,041 | 21,593,059 | CGGTTAAGCCAGGGCTGTG |
| TUP | 21,593,061 | 21,593,085 | ACGCGACTGAGTGAGTAGTGGCAGA |
| TUR | 21,593,117 | 21,593,134 | AGCTGCGGTGACAAGGAC |
| TDF | 21,593,663 | 21,593,683 | AGCTGCACTCAGAGGCCAATG |
| TDP | 21,593,684 | 21,593,711 | AGAGGCTGTTCGAGGAGAAGTACAAGCC |
| TDR | 21,593,715 | 21,593,736 | CACCTGATCGTCGGTGAGTATG |
PCR Primers
| Primer | Sequence |
| SU | TGCATGGAGCCTAATACACG |
| SD | AACAGGACTAATGCATGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12879A-H2 | VGB6 | C57BL/6NTac | |
| 12879A-G4 | VGB6 | C57BL/6NTac | |
| 12879A-F11 | VGB6 | C57BL/6NTac | |
| 12879A-E6 | VGB6 | C57BL/6NTac | |
