12880   Mettl7b     Status: ES cell colonies screened / QC positives

GeneMettl7b
Chromosome10
Deletion Start128,960,935
Deletion End128,958,688
Deletion Size2,248
Genome Build38
MGI IDMGI:1918914
NCBI Gene ID71664
BAC ID122K22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,960,300128,960,317TGCCTCTGCTGACGGTTG
TUP128,960,269128,960,294TCTGACCATGAAGGAGCACCGTAGGT
TUR128,960,173128,960,194TCCTCCACACCATGATAACTTC
TDF128,958,929128,958,950CCTACTGTTCTTCTGGGAGCAC
TDP128,958,897128,958,916CAGGGAAGCCGGGCCTTCCT
TDR128,958,847128,958,865GGCAACCATCTCCGATGTG

PCR Primers

PrimerSequence
SUACAGTAAGTCTGCTCTGCTC
SDGGGCCACATGGTGTAGTTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR145 bp++-
TDFTDR104 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12880A-C7VGB6C57BL/6NTac
12880A-G5VGB6C57BL/6NTac
12880A-F1VGB6C57BL/6NTac
12880A-B6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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