| Gene | Utp3 |
| Chromosome | 5 |
| Deletion Start | 88,554,617 |
| Deletion End | 88,556,023 |
| Deletion Size | 1,407 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,554,744 | 88,554,766 | ACCAAGACCAGGTAGATGAGTTC |
| TUP | 88,554,767 | 88,554,787 | CATGAAGCGCGATCTCGGGCA |
| TUR | 88,554,802 | 88,554,822 | CCACTCTCGACTTCGTTCCAG |
| TDF | 88,555,496 | 88,555,520 | GAAAGGCTTGTTACCTACAGAAATC |
| TDP | 88,555,524 | 88,555,550 | TCAACAAGCTGTCGGTTGTGGATCAGA |
| TDR | 88,555,577 | 88,555,597 | AGCACCATCCTTGGCTGTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGCCTAGCGTTCCTACC |
| SD | GGTTTTCATTGTCTCCACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12883A-B8 | VGB6 | C57BL/6NTac | |
| 12883A-E5 | VGB6 | C57BL/6NTac | |
| 12883A-H7 | VGB6 | C57BL/6NTac | |
| 12883A-F9 | VGB6 | C57BL/6NTac | |
