| Gene | 2010317E24Rik |
| Chromosome | 2 |
| Deletion Start | 25,372,434 |
| Deletion End | 25,376,540 |
| Deletion Size | 4,107 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,372,988 | 25,373,007 | CGCGGATTTGGGTGAGTTTG |
| TUP | 25,373,010 | 25,373,037 | TCCCTAGTCACACCTCGTAATTCCAGGG |
| TUR | 25,373,048 | 25,373,066 | CCTGTGGGTCTCCAGTTTC |
| TDF | 25,376,346 | 25,376,366 | GCCCAGGAGGTGACTGATAAG |
| TDP | 25,376,367 | 25,376,388 | AGTGAGCGCATCACGCAGCTGG |
| TDR | 25,376,396 | 25,376,416 | TGCTTGATGAGTGCAGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CCGTTTCTCGTTAAGTCTCC |
| SD | TGGTGGACAGAAGGAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12884A-G3 | VGB6 | C57BL/6NTac | |
| 12884A-D2 | VGB6 | C57BL/6NTac | |
| 12884A-E4 | VGB6 | C57BL/6NTac | |
