12889 1700034I23Rik Status: Cancelled
| Gene | 1700034I23Rik |
| Chromosome | 3 |
| Deletion Start | 40,902,435 |
| Deletion End | 40,901,859 |
| Deletion Size | 577 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,855,175 | 40,855,194 | CAACTGTGGCACGCTCTATC |
| TUP | 40,855,146 | 40,855,174 | TCCTCCTTTACGGACCCTCATCATTTCCC |
| TUR | 40,855,075 | 40,855,096 | GATCCAGTGCCTCGTAAGCATC |
| TDF | 40,854,781 | 40,854,798 | TTCGGAAGAGCGCCCAAA |
| TDP | 40,854,751 | 40,854,779 | ACCAACTGAAGTCAAGAGCAAAGCTGAGG |
| TDR | 40,854,730 | 40,854,750 | TCTTCACAAAGCACACCACCT |
PCR Primers
| Primer | Sequence |
| SU | GCAACACTTCTAATGTGAAATCG |
| SD | ATTGTGCATCCCTCTTCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
