Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12891 4921501E09Rik Status: ES cell colonies screened / QC positives

Gene	4921501E09Rik
Chromosome	17
Deletion Start	33,067,823
Deletion End	33,065,079
Deletion Size	2,745
Genome Build	38

MGI ID	MGI:1921292
NCBI Gene ID	74042
BAC ID	398H5
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	33,067,616	33,067,634	TGGCATGGATCGCCTAGAG
TUP	33,067,582	33,067,611	TGATGCTCTCAAGAGGAAGCCATTGATGAC
TUR	33,067,545	33,067,569	CCTACCCTGGATTTGGTGAATAGAG
TDF	33,065,755	33,065,778	GAATGGAAGTGAATCTGGTGGAAC
TDP	33,065,723	33,065,751	TGATCCACTAAAGGCCAAGAAACAGGTGG
TDR	33,065,675	33,065,696	TGCTGGAAGAGTTTGGGTCATC

PCR Primers

Primer	Sequence
SU	GTCTCTTTCCAGGCTCCTCC
SD	AGAAATTCCTAGGCCCAAGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	90 bp	+	+	-
TDF	TDR	104 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12891A-C8	VGB6	C57BL/6NTac
12891A-E10	VGB6	C57BL/6NTac
12891A-F8	VGB6	C57BL/6NTac
12891A-H8	VGB6	C57BL/6NTac