12891   4921501E09Rik     Status: ES cell colonies screened / QC positives

Gene4921501E09Rik
Chromosome17
Deletion Start33,204,792
Deletion End33,202,048
Deletion Size2,745
Genome Build37
MGI IDMGI:1921292
NCBI Gene ID74042
BAC ID398H5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTGGCATGGATCGCCTAGAG
TUPTGATGCTCTCAAGAGGAAGCCATTGATGAC
TURCCTACCCTGGATTTGGTGAATAGAG
TDFGAATGGAAGTGAATCTGGTGGAAC
TDPTGATCCACTAAAGGCCAAGAAACAGGTGG
TDRTGCTGGAAGAGTTTGGGTCATC

PCR Primers

PrimerSequence
SUGTCTCTTTCCAGGCTCCTCC
SDAGAAATTCCTAGGCCCAAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12891A-C8VGB6C57BL/6NTac
12891A-E10VGB6C57BL/6NTac
12891A-F8VGB6C57BL/6NTac
12891A-H8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image