12896 Idnk Status: Cancelled
| Gene | Idnk |
| Chromosome | 13 |
| Deletion Start | 58,157,690 |
| Deletion End | 58,163,798 |
| Deletion Size | 6,109 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,157,894 | 58,157,913 | GCGACCCTTCTCAGCCTTAC |
| TUP | 58,157,915 | 58,157,936 | AACCTGGGCCTGGTGGCACCTC |
| TUR | 58,157,965 | 58,157,984 | GACGTTTGCCTCCTGGTTTG |
| TDF | 58,163,047 | 58,163,066 | ACGGGTGAGGAACTGATGTC |
| TDP | 58,163,067 | 58,163,096 | CGGGAATAGAACACTTGAGCCTAAGACTGC |
| TDR | 58,163,128 | 58,163,147 | AAGCAGTGGGCTAAGCAATC |
PCR Primers
| Primer | Sequence |
| SU | AGGAACTGGCTTCCAGAGTG |
| SD | CTGCACACTGCCAACCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
