| Gene | Psapl1 |
| Chromosome | 5 |
| Deletion Start | 36,204,069 |
| Deletion End | 36,205,649 |
| Deletion Size | 1,581 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,204,307 | 36,204,326 | TGAACCCAGGTGCTACAGAG |
| TUP | 36,204,337 | 36,204,366 | TGACATCAGTAATGAAGACCTGTGAGTGGC |
| TUR | 36,204,377 | 36,204,397 | TTGCACTTAGCTGAAGACTCC |
| TDF | 36,204,912 | 36,204,932 | GAGATGCCGAGGACGAATGAG |
| TDP | 36,204,933 | 36,204,955 | TTGCAGATGCAGTTGGGCCTGAC |
| TDR | 36,204,971 | 36,204,990 | CCAGCTCCTGGACCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCATTGTGGGATCAGTAG |
| SD | TCACAGCATGACAGGAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12900B-H6 | VGB6 | C57BL/6NTac | |
| 12900B-H1 | VGB6 | C57BL/6NTac | |
| 12900B-G3 | VGB6 | C57BL/6NTac | |
| 12900B-E7 | VGB6 | C57BL/6NTac | |
| 12900B-C3 | VGB6 | C57BL/6NTac | |
| 12900B-A1 | VGB6 | C57BL/6NTac | |
