| Gene | Wdr6 |
| Chromosome | 9 |
| Deletion Start | 108,578,590 |
| Deletion End | 108,572,892 |
| Deletion Size | 5,699 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,577,852 | 108,577,872 | CCTTTGGTGATGGTGGTGTTG |
| TUP | 108,577,817 | 108,577,844 | CAAGGACCCTTGGCACTGACAATTAGGC |
| TUR | 108,577,790 | 108,577,811 | GCTGAGGCTAATAATGGCTGAC |
| TDF | 108,573,619 | 108,573,638 | ACTGATCTGGCTGTCCTCTC |
| TDP | 108,573,596 | 108,573,617 | TGGCTTCCAGGAGGCTGATCCT |
| TDR | 108,573,572 | 108,573,592 | CTGCCATCTGTAGCTGCACTG |
PCR Primers
| Primer | Sequence |
| SU | TACAGCCTCCATGCTGCTC |
| SD | TTTGGTCAGATGTTTGCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12908A-A6 | VGB6 | C57BL/6NTac | |
| 12908A-E7 | VGB6 | C57BL/6NTac | |
| 12908A-H11 | VGB6 | C57BL/6NTac | |
