12914 AI413582 Status: ES cell colonies screened / QC no positives
| Gene | AI413582 |
| Chromosome | 17 |
| Deletion Start | 27,564,669 |
| Deletion End | 27,563,940 |
| Deletion Size | 730 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGAAGTGGGTCTTGAGACTG |
| TUP | | | CAGTGCTCGCCTCCCCAGCC |
| TUR | | | TGCACATACATTACCTGTAACAGAG |
| TDF | | | TGCAGTGGCTTGCTCTTC |
| TDP | | | TTCATGGCTGGCAGAGCAGCTACC |
| TDR | | | GTGTGGACGGTCATGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTCCATTTAGCCGTGC |
| SD | CCTCCCTGGTTTCTTATTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
