12930 Gpr82 Status: Cancelled
| Gene | Gpr82 |
| Chromosome | X |
| Deletion Start | 13,665,218 |
| Deletion End | 13,666,278 |
| Deletion Size | 1,061 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,665,604 | 13,665,626 | CACCTCATGCTACGAGAGAATGT |
| TUP | 13,665,631 | 13,665,659 | TGGTCACGTACTGAAAAGATTTCGCCAGC |
| TUR | 13,665,690 | 13,665,710 | TGACAAGCACAACTCCCCATA |
| TDF | 13,665,752 | 13,665,774 | ACAGAAGAAGGACAGAGCCAGTG |
| TDP | 13,665,776 | 13,665,801 | TACAATCGGCAGATGGAACTGGGAGC |
| TDR | 13,665,814 | 13,665,834 | GTTCCAATGAGACCCGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TACGAATGATAAATCTCAGGC |
| SD | AAAGCAGGCAAACCCATAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
