12931 Gpr151 Status: ES cell colonies screened / QC one positive
| Gene | Gpr151 |
| Chromosome | 18 |
| Deletion Start | 42,579,608 |
| Deletion End | 42,578,248 |
| Deletion Size | 1,361 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,579,257 | 42,579,277 | GGTTCATCTGCAAGTCCTCTG |
| TUP | 42,579,232 | 42,579,255 | CTGGTTCACCCACGTGTGCATGGC |
| TUR | 42,579,200 | 42,579,222 | TGGCTACCACAACAAATGTCAAG |
| TDF | 42,578,824 | 42,578,845 | TGCTAAGCACTGCAATCATCTC |
| TDP | 42,578,800 | 42,578,823 | TGCTCTTCTGTGGCTCCCAGAGTG |
| TDR | 42,578,755 | 42,578,774 | ATCGGCCCTCCAGCCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCAAAGGACTTGAATCAGCC |
| SD | GCAAGTGAAGAGAATTTACAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
