12934   9930021J03Rik     Status: ES cell colonies screened / QC positives

Gene9930021J03Rik
Chromosome19
Deletion Start29,792,027
Deletion End29,790,501
Deletion Size1,527
Genome Build37
MGI IDMGI:2444398
NCBI Gene ID240613
BAC ID318G24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 19

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCAACTACACAACCAACAGTGTCTC
TUPCCAAATGCTTGACACCAGCTTTGCAGA
TURGGCAAGGCAACAGTCACAG
TDFTCACCTCTTCCTCCAGTTGTATC
TDPTGCCAGTCAGAATCCTGCATCTGTC
TDRTCAGGCTTCTCTAAAGCAGATGAC

PCR Primers

PrimerSequence
SUCGCTTCATCCTCAGCTATTG
SDACATCCTTACACCAATGAATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12934A-A6VGB6C57BL/6NTac
12934A-C3VGB6C57BL/6NTac
12934A-H6VGB6C57BL/6NTac
12934A-D5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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