| Gene | 9930021J03Rik |
| Chromosome | 19 |
| Deletion Start | 29,717,534 |
| Deletion End | 29,716,008 |
| Deletion Size | 1,527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,717,129 | 29,717,152 | CAACTACACAACCAACAGTGTCTC |
| TUP | 29,717,102 | 29,717,128 | CCAAATGCTTGACACCAGCTTTGCAGA |
| TUR | 29,717,080 | 29,717,098 | GGCAAGGCAACAGTCACAG |
| TDF | 29,716,087 | 29,716,109 | TCACCTCTTCCTCCAGTTGTATC |
| TDP | 29,716,062 | 29,716,086 | TGCCAGTCAGAATCCTGCATCTGTC |
| TDR | 29,716,024 | 29,716,047 | TCAGGCTTCTCTAAAGCAGATGAC |
PCR Primers
| Primer | Sequence |
| SU | CGCTTCATCCTCAGCTATTG |
| SD | ACATCCTTACACCAATGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12934A-A6 | VGB6 | C57BL/6NTac | |
| 12934A-C3 | VGB6 | C57BL/6NTac | |
| 12934A-H6 | VGB6 | C57BL/6NTac | |
| 12934A-D5 | VGB6 | C57BL/6NTac | |
